Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPRENI0011(Renin-inhibitory peptide)

DFBP ID	DFBPRENI0011
Peptide sequence	EEDDA
Type	Native peptide
Peptide/Function name	Renin-inhibitory peptide

Function-activity relationship

Main bioactivity	Renin-inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Glu-Glu-Asp-Asp-Ala

Single-letter amino acid

EEDDA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
577 Da	577.49 Da ^c

Net charge

-4

Isoelectric point (pI)

2.83

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.4400^c

Hydrophilic residue ratio

20%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rapeseed
Precursor protein	Napin
Residue position	f(31-35)
Precursor protein(s) search	Source.1: DFBPPR0995 ---- Plant proteins ---- Transcription factor TDR Source.2: DFBPPR1792 ---- Plant proteins ---- Probable 3-ketoacyl-CoA synthase 20 Source.3: DFBPPR2001 ---- Plant proteins ---- Importin subunit alpha-1b Source.4: DFBPPR3710 ---- Plant proteins ---- Putative beta-glucosidase 15 Source.5: DFBPPR4510 ---- Plant proteins ---- Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic Source.6: DFBPPR4940 ---- Plant proteins ---- Protein STAY-GREEN, chloroplastic Source.7: DFBPPR5731 ---- Plant proteins ---- Rubisco accumulation factor 1, chloroplastic Source.8: DFBPPR17411 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPB Source.9: DFBPPR18318 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase BAP1 Source.10: DFBPPR18515 ---- Animal proteins ---- cAMP-dependent protein kinase type II-beta regulatory subunit Source.11: DFBPPR19650 ---- Animal proteins ---- Small G protein signaling modulator 3 Source.12: DFBPPR22593 ---- Animal proteins ---- UPF0688 protein C1orf174 homolog Source.13: DFBPPR9988 ---- Animal proteins ---- Vinculin Source.14: DFBPPR10354 ---- Animal proteins ---- DNA topoisomerase 2-beta Source.15: DFBPPR10732 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPB Source.16: DFBPPR11348 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX10 Source.17: DFBPPR12768 ---- Animal proteins ---- Pepsin-3 Source.18: DFBPPR15704 ---- Microorganism protein ---- Oxidation resistance protein 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Renin-inhibitory activity	The final rapeseed protein hydrolysate (FRPH) exhibited potent Renin (EC:3.4.23.15) inhibitory activity with an IC₅₀ value of 0.47 ± 0.05 mg/mL. The peptide EEDDA was isolated from the FRPH using electrodialysis with ultrafiltration membrane (EDUF) technology, so it was a potential renin-inhibitory peptide (data not shown).
Specific target protein(s)	Specific Target Protein(s): Renin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Rapeseed protein isolate was subjected to alcalase digestion to obtain a protein hydrolysate.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1415
[D2]	DFBPANHY0037
[D3]	DFBPMUFU0368

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	He R, Girgih AT, Rozoy E, Bazinet L, Ju XR, Aluko RE. Selective separation and concentration of antihypertensive peptides from rapeseed protein hydrolysate by electrodialysis with ultrafiltration membranes. Food Chem. 2016 Apr 15;197(Pt A):1008-14. PMID: 26617047 DOI: 10.1016/j.foodchem.2015.11.081
Other literature(s)	[1] He R , Alashi A , Malomo S A , et al. Antihypertensive and free radical scavenging properties of enzymatic rapeseed protein hydrolysates[J]. Food Chemistry, 2013, 141(1):153-159.
PubDate	2016