Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2218(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2218
Peptide sequence	KKFY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Lys-Phe-Tyr

Single-letter amino acid

KKFY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	584.71 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.18 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Marine
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica proteins (LJPs)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1159 ---- Plant proteins ---- Replication protein A 70 kDa DNA-binding subunit B Source.2: DFBPPR2268 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 7 Source.3: DFBPPR2716 ---- Plant proteins ---- Endoglucanase 1 Source.4: DFBPPR3189 ---- Plant proteins ---- Endoglucanase 13 Source.5: DFBPPR4833 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 56 Source.6: DFBPPR7724 ---- Plant proteins ---- Avenacosidase 2 Source.7: DFBPPR16932 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.8: DFBPPR17050 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.9: DFBPPR17051 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.10: DFBPPR17442 ---- Animal proteins ---- AP-3 complex subunit beta-1 Source.11: DFBPPR17567 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL1 Source.12: DFBPPR17853 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL3 Source.13: DFBPPR18180 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL2 Source.14: DFBPPR18612 ---- Animal proteins ---- Microtubule-associated protein RP/EB family member 2 Source.15: DFBPPR18622 ---- Animal proteins ---- CYFIP-related Rac1 interactor B Source.16: DFBPPR19535 ---- Animal proteins ---- Probable 18S rRNA (guanine-N(7))-methyltransferase Source.17: DFBPPR20575 ---- Animal proteins ---- Peroxiredoxin-like 2A Source.18: DFBPPR20980 ---- Animal proteins ---- Interferon-inducible GTPase 5 Source.19: DFBPPR8786 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.20: DFBPPR9564 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H2 Source.21: DFBPPR10043 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.22: DFBPPR10077 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.23: DFBPPR10393 ---- Animal proteins ---- Vitellogenin-1 Source.24: DFBPPR10958 ---- Animal proteins ---- Heat shock cognate protein HSP 90-beta Source.25: DFBPPR12407 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.26: DFBPPR12480 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.27: DFBPPR12870 ---- Animal proteins ---- Protein transport protein Sec16B Source.28: DFBPPR13147 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.29: DFBPPR13189 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.30: DFBPPR13715 ---- Animal proteins ---- Ceroid-lipofuscinosis neuronal protein 5 Source.31: DFBPPR15083 ---- Microorganism protein ---- tRNA (guanine-N(7)-)-methyltransferase Source.32: DFBPPR15610 ---- Microorganism protein ---- Inheritance of peroxisomes protein 2
Link-research Inductive analysis	Link 1: DFBPACEI1326----Laminaria japonica----Laminaria japonica hydrolysates

Biological/Functional activity & target protein

ACE-inhibitory activity	N.D
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Enzyme ratio (papain: alcalase: trypsin) 1:2:1, pH 8.0, temperature 60 degrees C, substrate concentration 1.7%, enzyme addition 4.4%, and hydrolysis time 5.1 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

These results demonstrated the potential applications of LJPs as a source of anti-hypertensive peptides and provided a reference for the industrialized anti-hypertensive productions.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kumagai Y, Toji K, Katsukura S, Morikawa R, Uji T, Yasui H, Shimizu T, Kishimura H. Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein. Mar Drugs. 2021 Apr 1;19(4):200. PMID: PMC8066288 DOI: 10.3390/md19040200
Other literature(s)	N.D
PubDate	2021