DFBP ID - DFBPHAPE0023(Haemolytic peptide)

DFBP ID DFBPHAPE0023 Peptide sequence GWGSFFKKAAHVGKHVGKAALTHYL Type Native peptide Peptide/Function name Haemolytic peptide, Pleurocidin from Pleuronectes americanus

Function-activity relationship

Main bioactivity Haemolytic activity Otheir bioactivity Antimicrobial activity [D1], Anticancer activity [D2], Multifunctional activity [D3]

Calculated physicochemical properties

Three-letter amino acid Gly-Trp-Gly-Ser-Phe-Phe-Lys-Lys-Ala-Ala-His-Val-Gly-Lys-His-Val-Gly-Lys-Ala-Ala-Leu-Thr-His-Tyr-Leu Single-letter amino acid GWGSFFKKAAHVGKHVGKAALTHYL Peptide length 26 Peptide mass Experimental mass Theoretical mass N.D 2711.16 Da c Net charge 0.00 c Isoelectric point (pI) 10.64 c IC50 N.D pIC50 N.D GRAVY -0.0680 c Hydrophilic residue ratio 60% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal, Fish, Marine Organism/Source Winter flounder Precursor protein Pleurocidin Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis Link 1: DFBPHAPE0022----Winter flounder (Pleuronectes americanus)----The skin-secreted mucous of the winter flounder.

Biological/Functional activity & target protein

Haemolytic activity The peptide showed haemolytic activity. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES NCC(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture Synthesis peptide

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information MIC value = 2.5-5 uM.

Database cross-references

DFBP [D1] DFBPAMIC0033, DFBPAMIC0171 [D2] DFBPANCA0657, DFBPANCA0658 [D3] DFBPMUFU0699

BIOPEP-UWM [D4] 7758, 7761

APD [D5] -

BioPepDB [D6] -

MBPDB [D7] -

Reference(s)

Primary literature Sung WS, Lee DG. Pleurocidin-derived antifungal peptides with selective membrane-disruption effect. Biochem Biophys Res Commun. 2008 May 9;369(3):858-61. PMID: 18325325 DOI: 10.1016/j.bbrc.2008.02.109 Other literature(s) N.D PubDate 2008