Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0020(Antithrombotic peptide)

DFBP ID	DFBPANTH0020
Peptide sequence	RGDS
Type	Native peptide
Peptide/Function name	Antithrombotic peptide, Fibronectin inhibitor

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Gly-Asp-Ser

Single-letter amino acid

RGDS

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	433.41 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.74 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.3000^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human fibrinogen^[3]
Precursor protein	α-Chain
Residue position	f(572-575)
Precursor protein(s) search	Source.1: DFBPPR0953 ---- Plant proteins ---- Hexokinase-5 Source.2: DFBPPR1394 ---- Plant proteins ---- Lipoxygenase 7, chloroplastic Source.3: DFBPPR1533 ---- Plant proteins ---- Chaperone protein ClpB1 Source.4: DFBPPR1726 ---- Plant proteins ---- SPX domain-containing protein 1 Source.5: DFBPPR1891 ---- Plant proteins ---- Transcription factor MYBS2 Source.6: DFBPPR1918 ---- Plant proteins ---- DNA replication licensing factor MCM6 Source.7: DFBPPR1922 ---- Plant proteins ---- Cyclin-dependent kinase G-2 Source.8: DFBPPR2166 ---- Plant proteins ---- Eukaryotic translation initiation factor isoform 4G-1 Source.9: DFBPPR2801 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 21 Source.10: DFBPPR2828 ---- Plant proteins ---- ABC transporter G family member 36 Source.11: DFBPPR2886 ---- Plant proteins ---- 60S acidic ribosomal protein P0 Source.12: DFBPPR3099 ---- Plant proteins ---- Putative GTP diphosphokinase RSH1, chloroplastic Source.13: DFBPPR3121 ---- Plant proteins ---- Endoglucanase 23 Source.14: DFBPPR3189 ---- Plant proteins ---- Endoglucanase 13 Source.15: DFBPPR3265 ---- Plant proteins ---- Probable protein phosphatase 2C 18 Source.16: DFBPPR3307 ---- Plant proteins ---- Endoglucanase 18 Source.17: DFBPPR3377 ---- Plant proteins ---- Endoglucanase 3 Source.18: DFBPPR3493 ---- Plant proteins ---- Endoglucanase 20 Source.19: DFBPPR3667 ---- Plant proteins ---- Probable NAD kinase 2, chloroplastic Source.20: DFBPPR3887 ---- Plant proteins ---- Endoglucanase 8 Source.21: DFBPPR3888 ---- Plant proteins ---- Endoglucanase 24 Source.22: DFBPPR4296 ---- Plant proteins ---- Squamosa promoter-binding-like protein 6 Source.23: DFBPPR4361 ---- Plant proteins ---- Formin-like protein 8 Source.24: DFBPPR5248 ---- Plant proteins ---- 60S acidic ribosomal protein P0 Source.25: DFBPPR5613 ---- Plant proteins ---- 60S acidic ribosomal protein P0 Source.26: DFBPPR6328 ---- Plant proteins ---- Magnesium-chelatase subunit ChlD, chloroplastic Source.27: DFBPPR7492 ---- Plant proteins ---- Thioredoxin F-type, chloroplastic Source.28: DFBPPR7606 ---- Milk proteins ---- Osteopontin Source.29: DFBPPR8491 ---- Milk proteins ---- Osteopontin Source.30: DFBPPR16092 ---- Animal proteins ---- Tight junction protein ZO-2 Source.31: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.32: DFBPPR16836 ---- Animal proteins ---- Fibronectin Source.33: DFBPPR16842 ---- Animal proteins ---- Collagen alpha-1(II) chain Source.34: DFBPPR16847 ---- Animal proteins ---- Fibrinogen alpha chain Source.35: DFBPPR16920 ---- Animal proteins ---- Zona pellucida sperm-binding protein 2 Source.36: DFBPPR17358 ---- Animal proteins ---- Aldo-keto reductase family 1 member A1 Source.37: DFBPPR17442 ---- Animal proteins ---- AP-3 complex subunit beta-1 Source.38: DFBPPR17757 ---- Animal proteins ---- NAD(P)H-hydrate epimerase Source.39: DFBPPR18481 ---- Animal proteins ---- Plasma kallikrein Source.40: DFBPPR18791 ---- Animal proteins ---- Small nuclear ribonucleoprotein Sm D2 Source.41: DFBPPR18999 ---- Animal proteins ---- Keratin, type II cytoskeletal 74 Source.42: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.43: DFBPPR19135 ---- Animal proteins ---- Palmitoyltransferase ZDHHC5 Source.44: DFBPPR19462 ---- Animal proteins ---- Intercellular adhesion molecule 1 Source.45: DFBPPR19924 ---- Animal proteins ---- Protocadherin-18 Source.46: DFBPPR20511 ---- Animal proteins ---- Mitoguardin 2 Source.47: DFBPPR20651 ---- Animal proteins ---- Succinate dehydrogenase assembly factor 2, mitochondrial Source.48: DFBPPR20778 ---- Animal proteins ---- Tetraspanin-15 Source.49: DFBPPR20944 ---- Animal proteins ---- Pikachurin Source.50: DFBPPR21556 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit gamma Source.51: DFBPPR21705 ---- Animal proteins ---- Transmembrane protein 50B Source.52: DFBPPR21980 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein Source.53: DFBPPR22339 ---- Animal proteins ---- R3H domain-containing protein 2 Source.54: DFBPPR22421 ---- Animal proteins ---- Placenta-specific protein 9 Source.55: DFBPPR22503 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein-like Source.56: DFBPPR8656 ---- Animal proteins ---- Chromogranin-A Source.57: DFBPPR8806 ---- Animal proteins ---- Cadherin-5 Source.58: DFBPPR8903 ---- Animal proteins ---- Zona pellucida sperm-binding protein 2 Source.59: DFBPPR8904 ---- Animal proteins ---- Osteopontin Source.60: DFBPPR9591 ---- Animal proteins ---- Bone sialoprotein 2 Source.61: DFBPPR9937 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein Source.62: DFBPPR9983 ---- Animal proteins ---- Fibrinogen alpha chain Source.63: DFBPPR10350 ---- Animal proteins ---- Protein dispatched homolog 3 Source.64: DFBPPR10355 ---- Animal proteins ---- Down syndrome cell adhesion molecule-like protein 1 homolog Source.65: DFBPPR10530 ---- Animal proteins ---- Osteopontin Source.66: DFBPPR10541 ---- Animal proteins ---- Glypican-1 Source.67: DFBPPR10604 ---- Animal proteins ---- Integrin alpha-8 Source.68: DFBPPR10635 ---- Animal proteins ---- Dual serine/threonine and tyrosine protein kinase Source.69: DFBPPR10654 ---- Animal proteins ---- Fibronectin Source.70: DFBPPR11018 ---- Animal proteins ---- Transcriptional coactivator YAP1 Source.71: DFBPPR11044 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.72: DFBPPR11329 ---- Animal proteins ---- Bone sialoprotein 2 Source.73: DFBPPR11350 ---- Animal proteins ---- Homeobox protein Hox-D13 Source.74: DFBPPR12128 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein-like Source.75: DFBPPR12218 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein homolog Source.76: DFBPPR12310 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1C Source.77: DFBPPR12386 ---- Animal proteins ---- Vitamin K-dependent protein C Source.78: DFBPPR12453 ---- Animal proteins ---- Lactase-phlorizin hydrolase Source.79: DFBPPR12602 ---- Animal proteins ---- Osteopontin Source.80: DFBPPR12603 ---- Animal proteins ---- Osteopontin Source.81: DFBPPR13115 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein Source.82: DFBPPR13185 ---- Animal proteins ---- Chromogranin-A Source.83: DFBPPR13419 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein Source.84: DFBPPR13704 ---- Animal proteins ---- Osteopontin Source.85: DFBPPR13969 ---- Animal proteins ---- Suppressor of tumorigenicity 7 protein Source.86: DFBPPR14187 ---- Marine protein ---- Secreted phosphoprotein 24 Source.87: DFBPPR14716 ---- Marine protein ---- Secreted phosphoprotein 24 Source.88: DFBPPR15031 ---- Microorganism protein ---- NAD-dependent histone deacetylase SIR2 Source.89: DFBPPR15135 ---- Microorganism protein ---- Protein transport protein SEC22 Source.90: DFBPPR15750 ---- Microorganism protein ---- 60S ribosomal protein L24 Source.91: DFBPPR7910 ---- Plant protein ---- Glycine-rich RNA-binding protein 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	The fibrinogen tetrapeptide RGDS effectively inhibited ADP-induced human platelet aggregation (IC₅₀ = 75 μM) and ADP-induced [¹²⁵I]fibrinogen binding to human platelets (IC₅₀ = 20 μM)^{[2, 4]}.
Specific target protein(s)	Potential Target Protein(s): Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4 Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CO)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide showed no inhibition on binding of monoclonal antibodies to platelet glycoproteins (GPIIb-GPIIIa)^[2].

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Pierschbacher MD, Ruoslahti E. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature. 1984 May 3-9;309(5963):30-3. PMID: 6325925 DOI: 10.1038/309030a0
Other literature(s)	[1] Meddeb S, Demaret J P, Ballini J P, et al. A conformational study of Lys-Arg-Asp-Ser and analogs, a series of potent antithrombotic peptides. An approach based on simulated annealing and 1H NMR.[J]. Journal of Biomolecular Structure & Dynamics, 1994, 11(5):959. [2] Fiat A M, Levytoledano S, Caen J P, et al. Biologically active peptides of casein and lactotransferrin implicated in platelet function.[J]. Journal of Dairy Research, 1989, 56(3):351-355. [3] Mazoyer E, Lévy-Toledano S, Rendu F, et al. KRDS, a new peptide derived from human lactotransferrin, inhibits platelet aggregation and release reaction.[J]. Febs Journal, 1990, 194(1):43-49. [4] Fiat A M, Migliore-Samour D, Jollã¨S P, et al. Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities[J]. Journal of Dairy Science, 1993, 76(1):301-310.
PubDate	1984