Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0100(Antithrombotic peptide)

DFBP ID	DFBPANTH0100
Peptide sequence	LCRR
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Cys-Arg-Arg

Single-letter amino acid

LCRR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
552 Da	546.68 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.89 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine
Organism/Source	Goby mussel (Zosterisessor ophiocephalus)
Precursor protein	Muscle proteins
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0955 ---- Plant proteins ---- Gibberellin receptor GID1 Source.2: DFBPPR1078 ---- Plant proteins ---- Sucrose transport protein SUT5 Source.3: DFBPPR1108 ---- Plant proteins ---- Tricin synthase 2 Source.4: DFBPPR1809 ---- Plant proteins ---- Chaperone protein ClpB3, mitochondrial Source.5: DFBPPR2354 ---- Plant proteins ---- Thioredoxin-like protein CDSP32, chloroplastic Source.6: DFBPPR2369 ---- Plant proteins ---- Kinesin-like protein KIN-UB Source.7: DFBPPR2868 ---- Plant proteins ---- Probable 4-coumarate--CoA ligase 2 Source.8: DFBPPR3023 ---- Plant proteins ---- RNA pseudouridine synthase 6, chloroplastic Source.9: DFBPPR3129 ---- Plant proteins ---- Protein disulfide isomerase-like 5-4 Source.10: DFBPPR3193 ---- Plant proteins ---- Potassium channel AKT3 Source.11: DFBPPR3331 ---- Plant proteins ---- RNA pseudouridine synthase 3, mitochondrial Source.12: DFBPPR3399 ---- Plant proteins ---- Mannan endo-1,4-beta-mannosidase 4 Source.13: DFBPPR3576 ---- Plant proteins ---- Transport inhibitor response 1-like protein Os11g0515500 Source.14: DFBPPR3838 ---- Plant proteins ---- Probable protein phosphatase 2C 47 Source.15: DFBPPR3940 ---- Plant proteins ---- Putative cyclin-D2-3 Source.16: DFBPPR4377 ---- Plant proteins ---- Trafficking protein particle complex II-specific subunit 120 homolog Source.17: DFBPPR4736 ---- Plant proteins ---- B3 domain-containing protein Os04g0581400 Source.18: DFBPPR4844 ---- Plant proteins ---- B3 domain-containing protein Os05g0481400 Source.19: DFBPPR4890 ---- Plant proteins ---- NAC domain-containing protein 48 Source.20: DFBPPR5138 ---- Plant proteins ---- Subtilisin-like protease Glyma18g48580 Source.21: DFBPPR5440 ---- Plant proteins ---- Adenylyltransferase and sulfurtransferase MOCS3-1 Source.22: DFBPPR5459 ---- Plant proteins ---- Adenylyltransferase and sulfurtransferase MOCS3-2 Source.23: DFBPPR5770 ---- Plant proteins ---- Caffeoyl-CoA O-methyltransferase 1 Source.24: DFBPPR5775 ---- Plant proteins ---- Caffeoyl-CoA O-methyltransferase 2 Source.25: DFBPPR6048 ---- Plant proteins ---- CASP-like protein 5B1 Source.26: DFBPPR16010 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, muscle type Source.27: DFBPPR16050 ---- Animal proteins ---- Dual oxidase 1 Source.28: DFBPPR16233 ---- Animal proteins ---- Signal recognition particle subunit SRP72 Source.29: DFBPPR16368 ---- Animal proteins ---- Tyrosinase Source.30: DFBPPR16973 ---- Animal proteins ---- Fatty acid synthase Source.31: DFBPPR17083 ---- Animal proteins ---- Cyclin-dependent kinase 5 activator 1 Source.32: DFBPPR17392 ---- Animal proteins ---- Carbonyl reductase [NADPH] 1 Source.33: DFBPPR17509 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, muscle type Source.34: DFBPPR17604 ---- Animal proteins ---- Regulator of telomere elongation helicase 1 Source.35: DFBPPR18395 ---- Animal proteins ---- Galactosylceramide sulfotransferase Source.36: DFBPPR18875 ---- Animal proteins ---- S-adenosylmethionine synthase isoform type-1 Source.37: DFBPPR19061 ---- Animal proteins ---- RNA-binding protein 5 Source.38: DFBPPR19285 ---- Animal proteins ---- Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Source.39: DFBPPR19382 ---- Animal proteins ---- Arrestin-C Source.40: DFBPPR19476 ---- Animal proteins ---- Rho GTPase-activating protein 7 Source.41: DFBPPR19933 ---- Animal proteins ---- Oligoribonuclease, mitochondrial Source.42: DFBPPR20434 ---- Animal proteins ---- SPRY domain-containing SOCS box protein 1 Source.43: DFBPPR20574 ---- Animal proteins ---- Transmembrane protein 201 Source.44: DFBPPR20781 ---- Animal proteins ---- Proline dehydrogenase 1, mitochondrial Source.45: DFBPPR20877 ---- Animal proteins ---- BTB/POZ domain-containing protein KCTD5 Source.46: DFBPPR20901 ---- Animal proteins ---- [Protein ADP-ribosylarginine] hydrolase-like protein 1 Source.47: DFBPPR21018 ---- Animal proteins ---- Solute carrier family 22 member 17 Source.48: DFBPPR21192 ---- Animal proteins ---- Oxidation resistance protein 1 Source.49: DFBPPR21405 ---- Animal proteins ---- 60S ribosomal protein L37 Source.50: DFBPPR21474 ---- Animal proteins ---- m-AAA protease-interacting protein 1, mitochondrial Source.51: DFBPPR21590 ---- Animal proteins ---- Rhombotin-1 Source.52: DFBPPR21702 ---- Animal proteins ---- Rhombotin-2 Source.53: DFBPPR21761 ---- Animal proteins ---- NADH dehydrogenase (ubiquinone) complex I, assembly factor 6 Source.54: DFBPPR21962 ---- Animal proteins ---- Tetraspanin-3 Source.55: DFBPPR22357 ---- Animal proteins ---- Transmembrane protein 180 Source.56: DFBPPR22547 ---- Animal proteins ---- Actin-related protein T2 Source.57: DFBPPR22559 ---- Animal proteins ---- Vimentin-type intermediate filament-associated coiled-coil protein Source.58: DFBPPR22641 ---- Animal proteins ---- LIM domain only protein 3 Source.59: DFBPPR8577 ---- Animal proteins ---- Nectin-1 Source.60: DFBPPR8754 ---- Animal proteins ---- Dual oxidase 1 Source.61: DFBPPR8780 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, muscle type Source.62: DFBPPR9956 ---- Animal proteins ---- Fatty acid synthase Source.63: DFBPPR10162 ---- Animal proteins ---- Dynamin-like 120 kDa protein, mitochondrial Source.64: DFBPPR10227 ---- Animal proteins ---- Serine/threonine-protein kinase STK11 Source.65: DFBPPR10236 ---- Animal proteins ---- Acid-sensing ion channel 1 Source.66: DFBPPR10671 ---- Animal proteins ---- Cilia- and flagella-associated protein 65 Source.67: DFBPPR11984 ---- Animal proteins ---- SET and MYND domain-containing protein 4 Source.68: DFBPPR12211 ---- Animal proteins ---- Transmembrane protein 180 Source.69: DFBPPR12237 ---- Animal proteins ---- Thyroid adenoma-associated protein homolog Source.70: DFBPPR12244 ---- Animal proteins ---- Cysteine-rich DPF motif domain-containing protein 1 Source.71: DFBPPR12342 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, muscle type Source.72: DFBPPR12343 ---- Animal proteins ---- Protein SLFN14 Source.73: DFBPPR12365 ---- Animal proteins ---- Sucrase-isomaltase, intestinal Source.74: DFBPPR13183 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, muscle type Source.75: DFBPPR14157 ---- Marine protein ---- Ribosome biogenesis protein wdr12 Source.76: DFBPPR14162 ---- Marine protein ---- Pescadillo homolog Source.77: DFBPPR14761 ---- Marine protein ---- Intracellular coagulation inhibitor 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	The peptide could potently prolonged the thrombin time (TT) and the activated partial thromboplastin time (APTT) in a dose-dependant manner. This result implies that the peptide extracts inhibit coagulation factors such as FXIIa, FXIa, tenase components (FIXa, FVIIIa, FX, Ca2+, phospholipids) in the intrinsic blood coagulation pathway ^[2].
Specific target protein(s)	Specific Target Protein(s): Coagulation factor IX Coagulation factor VIII Coagulation factor X Coagulation factor XI Coagulation factor XII

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The hydrolysate generated by the crude protease from Bacillus licheniformis NH1 displayed the highest anticoagulant activity.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Goby protein hydrolysates may potentially serve as a good source of anticoagulant peptides that could be utilised to develop functional foods for thrombosis prevention.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Nasri, R., Amor, I.B., Bougatef, A., Nedjar-Arroume, N., Dhulster, P., Gargouri, J., et al. Anticoagulant activities of goby muscle protein hydrolysates. Food Chem. 2012, 133, 835-41. DOI: 10.1016/j.foodchem.2012.01.101
Other literature(s)	[1] Cheng, S., et al., Food-derived antithrombotic peptides: Preparation, identification, and interactions with thrombin. Crit Rev Food Sci Nutr, 2019: p. 1-15. [2] Jo H Y , Jung W K , Kim S K . Purification and characterization of a novel anticoagulant peptide from marine echiuroid worm, Urechis unicinctus[J]. Process Biochemistry, 2008, 43(2):179-184.
PubDate	2012