Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0002(Antithrombotic peptide)

DFBP ID	DFBPANTH0002
Peptide sequence	KDQDK
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Asp-Gln-Asp-Lys

Single-letter amino acid

KDQDK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	632.67 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.83 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-3.6600^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Sheep milk protein
Precursor protein	κ-Casein
Residue position	f(112-116)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR18903 ---- Animal proteins ---- Glutaryl-CoA dehydrogenase, mitochondrial Source.4: DFBPPR8916 ---- Animal proteins ---- Glutaryl-CoA dehydrogenase, mitochondrial Source.5: DFBPPR15004 ---- Microorganism protein ---- Ubiquitin-conjugating enzyme E2 6
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	KDQDK completely inhibited thrombin-induced platelet aggregation (100% inhibition).
Specific target protein(s)	Potential Target Protein(s): Integrin beta-3 Integrin alpha-IIb Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	All sheep caseinoglycopeptide (CGP, sheep κ-casein: 106-171) used was prepared with sheep caseinate treated by chymosin, and then the CGP was digested with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Qian, Z.-Y., Jollès, P., Migliore-Samour, D., Schoentgen, F., Fiat, A.-M. Sheep κ-casein peptides inhibit platelet aggregation. Biochimica et Biophysica Acta (BBA) - General Subjects. 1995, 1244, 411-7. DOI: 10.1016/0304-4165(95)00047-F
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009. [2] Fosset S, Tomé D. Dietary protein-derived peptides with antithrombotic activity.[J]. Bulletin of the International Dairy Federation, 2000.
PubDate	1995