Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0053(Antithrombotic peptide)

DFBP ID	DFBPANTH0053
Peptide sequence	MAIPPKK
Type	Native peptide
Peptide/Function name	Antithrombotic peptide, Casoplatelin

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Met-Ala-Ile-Pro-Pro-Lys-Lys

Single-letter amino acid

MAIPPKK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	784.02 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.59 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4000^c

Hydrophilic residue ratio

71.43%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Sheep milk protein
Precursor protein	κ-Casein
Residue position	f(106-112)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	Link 1: DFBPANTH0006----Bovine milk protein----κ-Casein Link 2: DFBPANTH0162----Bovine milk (Bos taurus)----κ-Casein

Biological/Functional activity & target protein

Antithrombotic activity

MAIPPKK weakly inhibited thrombin-induced platelet aggregation (10% inhibition). The peptide is inhibitors of both, the aggregation of ADP-activated platelets and binding of human fibrinogen γ-chain to a specific receptor region on the platelet sufrace^[3]. The interacting region of the fibrinogen γ-chain in platelet aggregation is the C-terminal dodecapeptide sequence that itself possesses similar inhibiting effects ^[4].

Specific target protein(s)

Specific Target Protein(s):
Fibrinogen gamma chain
Potential Target Protein(s):
Integrin beta-3
Integrin alpha-IIb
Integrin alpha-2
Platelet glycoprotein IX
Platelet glycoprotein Ib alpha chain
Platelet glycoprotein Ib beta chain
Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	All sheep caseinoglycopeptide (CGP, sheep κ-casein: 106-171) used was prepared with sheep caseinate treated by chymosin, and then the CGP was digested with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide was also found in Bovine milk proteins ^{[D2, 2]}.

Database cross-references

DFBP

[D1]	DFBPACEI0291
[D2]	DFBPANHY0543
[D3]	DFBPMUFU0393

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Qian, Z.-Y., Jollès, P., Migliore-Samour, D., Schoentgen, F., Fiat, A.-M. Sheep κ-casein peptides inhibit platelet aggregation. Biochimica et Biophysica Acta (BBA) - General Subjects. 1995, 1244, 411-7. DOI: 10.1016/0304-4165(95)00047-F
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009. [2] Qian Z Y, Jollès P, Miglioresamour D, et al. Sheep kappa-casein peptides inhibit platelet aggregation.[J]. Biochimica Et Biophysica Acta, 1995, 1244(2–3):411-417. [3] Fiat A M, Levytoledano S, Caen J P, et al. Biologically active peptides of casein and lactotransferrin implicated in platelet function.[J]. Journal of Dairy Research, 1989, 56(3):351-355. [4] Kloczewiak M, Timmons S, Lukas T J, et al. Platelet receptor recognition site on human fibrinogen. Synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the gamma chain[J]. Biochemistry, 1984, 23(8):1767-74.
PubDate	1995