Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0059(Antithrombotic peptide)

DFBP ID	DFBPANTH0059
Peptide sequence	KRDR
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Arg-Asp-Arg

Single-letter amino acid

KRDR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	573.64 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.38 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-4.1000^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Pisum sativum (Garden pea)
Precursor protein	Convicilin
Residue position	f(178-181)
Precursor protein(s) search	Source.1: DFBPPR1268 ---- Plant proteins ---- Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic Source.2: DFBPPR1330 ---- Plant proteins ---- Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic Source.3: DFBPPR1602 ---- Plant proteins ---- SNW/SKI-interacting protein A Source.4: DFBPPR1911 ---- Plant proteins ---- Heat stress transcription factor A-6a Source.5: DFBPPR2495 ---- Plant proteins ---- Probable histone acetyltransferase HAC-like 3 Source.6: DFBPPR2544 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.7: DFBPPR2859 ---- Plant proteins ---- Proton pump-interactor BIP131 Source.8: DFBPPR3374 ---- Plant proteins ---- Auxin-responsive protein IAA19 Source.9: DFBPPR3425 ---- Plant proteins ---- Transcription initiation factor TFIID subunit 1 Source.10: DFBPPR3557 ---- Plant proteins ---- NRR repressor homolog 3 Source.11: DFBPPR3580 ---- Plant proteins ---- Ribosome biogenesis protein BOP1 homolog Source.12: DFBPPR4438 ---- Plant proteins ---- Pre-mRNA-splicing factor SLU7 Source.13: DFBPPR5076 ---- Plant proteins ---- Xyloglucan endotransglucosylase/hydrolase 1 Source.14: DFBPPR5760 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.15: DFBPPR6453 ---- Plant proteins ---- Convicilin Source.16: DFBPPR6458 ---- Plant proteins ---- Convicilin Source.17: DFBPPR6760 ---- Plant proteins ---- Probable xyloglucan endotransglucosylase/hydrolase Source.18: DFBPPR6830 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.19: DFBPPR7070 ---- Plant proteins ---- Red chlorophyll catabolite reductase Source.20: DFBPPR7208 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.21: DFBPPR8466 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.22: DFBPPR16199 ---- Animal proteins ---- Potassium-transporting ATPase alpha chain 1 Source.23: DFBPPR16264 ---- Animal proteins ---- Pancreatic prohormone Source.24: DFBPPR16548 ---- Animal proteins ---- Coiled-coil domain-containing protein 39 Source.25: DFBPPR17329 ---- Animal proteins ---- Steroidogenic factor 1 Source.26: DFBPPR17411 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPB Source.27: DFBPPR18024 ---- Animal proteins ---- Kynurenine--oxoglutarate transaminase 3 Source.28: DFBPPR18429 ---- Animal proteins ---- Pre-mRNA-splicing factor SLU7 Source.29: DFBPPR18737 ---- Animal proteins ---- Centrosomal protein of 120 kDa Source.30: DFBPPR19532 ---- Animal proteins ---- Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 Source.31: DFBPPR20192 ---- Animal proteins ---- Microfibrillar-associated protein 1 Source.32: DFBPPR20287 ---- Animal proteins ---- Large neutral amino acids transporter small subunit 4 Source.33: DFBPPR20650 ---- Animal proteins ---- WD repeat-containing protein 91 Source.34: DFBPPR20719 ---- Animal proteins ---- DnaJ homolog subfamily C member 21 Source.35: DFBPPR21528 ---- Animal proteins ---- Vasculin Source.36: DFBPPR21647 ---- Animal proteins ---- U11/U12 small nuclear ribonucleoprotein 35 kDa protein Source.37: DFBPPR22269 ---- Animal proteins ---- Protein preY, mitochondrial Source.38: DFBPPR22532 ---- Animal proteins ---- Cilia- and flagella-associated protein 299 Source.39: DFBPPR8600 ---- Animal proteins ---- Steroidogenic factor 1 Source.40: DFBPPR8783 ---- Animal proteins ---- Potassium-transporting ATPase alpha chain 1 Source.41: DFBPPR10351 ---- Animal proteins ---- Protein sidekick-2 Source.42: DFBPPR10415 ---- Animal proteins ---- Semaphorin-3A Source.43: DFBPPR11224 ---- Animal proteins ---- Nuclear receptor subfamily 5 group A member 2 Source.44: DFBPPR11458 ---- Animal proteins ---- Sarcalumenin Source.45: DFBPPR11704 ---- Animal proteins ---- Pre-mRNA-splicing factor SLU7 Source.46: DFBPPR11747 ---- Animal proteins ---- Microfibrillar-associated protein 1 Source.47: DFBPPR11804 ---- Animal proteins ---- WD repeat-containing protein 91 Source.48: DFBPPR11937 ---- Animal proteins ---- Bromodomain-containing protein 7 Source.49: DFBPPR12068 ---- Animal proteins ---- Serine/arginine repetitive matrix protein 1 Source.50: DFBPPR12654 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.51: DFBPPR13285 ---- Animal proteins ---- Steroidogenic factor 1 Source.52: DFBPPR13716 ---- Animal proteins ---- Trichohyalin Source.53: DFBPPR14291 ---- Marine protein ---- Photosystem II D2 protein Source.54: DFBPPR14338 ---- Marine protein ---- Photosystem II D2 protein Source.55: DFBPPR14892 ---- Microorganism protein ---- Histone-lysine N-methyltransferase, H3 lysine-4 specific Source.56: DFBPPR14971 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP2 Source.57: DFBPPR15134 ---- Microorganism protein ---- Eukaryotic translation initiation factor 3 subunit A Source.58: DFBPPR15217 ---- Microorganism protein ---- GPI mannosyltransferase 1 Source.59: DFBPPR15593 ---- Microorganism protein ---- SWR1-complex protein 3 Source.60: DFBPPR15635 ---- Microorganism protein ---- Pre-mRNA-splicing factor SLU7 Source.61: DFBPPR15647 ---- Microorganism protein ---- Protein IBD2 Source.62: DFBPPR7816 ---- Plant protein ---- DNA-directed RNA polymerase subunit alpha Source.63: DFBPPR8034 ---- Plant protein ---- Linoleate 9S-lipoxygenase 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	Amino acid substitutions within the sequence KRDS modifies the biological activity: KRDY or KRDR have a reduced antithrombotic effect while KREE is more active than KRDS^[1].
Specific target protein(s)	Potential Target Protein(s): Integrin beta-3 Integrin alpha-IIb Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Mazoyer E, Lévy-Toledano S, Rendu F, Hermant L, Lu H, Fiat AM, Jollès P, Caen J. KRDS, a new peptide derived from human lactotransferrin, inhibits platelet aggregation and release reaction. Eur J Biochem. 1990 Nov 26;194(1):43-9. PMID: 2174781 DOI: 10.1111/j.1432-1033.1990.tb19424.x
Other literature(s)	[1] Meddeb S, Demaret J P, Ballini J P, et al. A conformational study of Lys-Arg-Asp-Ser and analogs, a series of potent antithrombotic peptides. An approach based on simulated annealing and 1H NMR.[J]. Journal of Biomolecular Structure & Dynamics, 1994, 11(5):959.
PubDate	1990