Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0067(Antithrombotic peptide)

DFBP ID	DFBPANTH0067
Peptide sequence	GPRPP
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Pro-Arg-Pro-Pro

Single-letter amino acid

GPRPP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	522.60 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.9400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig
Precursor protein	Porcine skin collagen
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR4863 ---- Plant proteins ---- Protein MOTHER of FT and TFL1 homolog 1 Source.2: DFBPPR17156 ---- Animal proteins ---- ATP-dependent RNA helicase A Source.3: DFBPPR17644 ---- Animal proteins ---- CCAAT/enhancer-binding protein beta Source.4: DFBPPR20083 ---- Animal proteins ---- GPN-loop GTPase 1
Link-research Inductive analysis	Link 1: DFBPANTH0008----Pig----Porcine skin collagen Link 2: DFBPANTH0081----Pig----Porcine skin collagen

Biological/Functional activity & target protein

Antithrombotic activity	The peptide inhibited platelet aggregation of rat and human PRP and the platelet aggregation was inhibited by more than 50% by GPRPP at a concentration of 0.3 mM. The effects of GPRPP was about 10 times higher in human PRP than in rat PRP.
Specific target protein(s)	Potential Target Protein(s): Integrin beta-3 Integrin alpha-IIb Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Collagen was hydrolyzed with collagenase or thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Nonaka I, Katsuda SI, Ohmori T, Shigehisa T, Nakagami T, Maruyama S. In Vitro and in Vivo Anti-platelet Effects of Enzymatic Hydrolysates of Collagen and Collagen-related Peptides. Biosci Biotechnol Biochem. 1997 Jan;61(5):772-775. PMID: 28862560 DOI: 10.1271/bbb.61.772
Other literature(s)	N.D
PubDate	1997