Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0038(Antithrombotic peptide)

DFBP ID	DFBPANTH0038
Peptide sequence	RGDF
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Gly-Asp-Phe

Single-letter amino acid

RGDF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	493.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.74 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Synthesis
Organism/Source	Synthesis
Precursor protein	Synthesis peptide
Residue position	f(95-98)
Precursor protein(s) search	Source.1: DFBPPR1211 ---- Plant proteins ---- Magnesium-chelatase subunit ChlD, chloroplastic Source.2: DFBPPR2014 ---- Plant proteins ---- Chaperone protein ClpB2, chloroplastic Source.3: DFBPPR2473 ---- Plant proteins ---- 2-hydroxyacyl-CoA lyase Source.4: DFBPPR2669 ---- Plant proteins ---- Arginine decarboxylase 1 Source.5: DFBPPR2695 ---- Plant proteins ---- Putative CBL-interacting protein kinase 27 Source.6: DFBPPR2913 ---- Plant proteins ---- DNA damage-binding protein 1 Source.7: DFBPPR2993 ---- Plant proteins ---- Beta-glucosidase 5 Source.8: DFBPPR3103 ---- Plant proteins ---- Beta-glucosidase 4 Source.9: DFBPPR3384 ---- Plant proteins ---- Putative coatomer subunit beta'-3 Source.10: DFBPPR3672 ---- Plant proteins ---- Coatomer subunit beta'-2 Source.11: DFBPPR4283 ---- Plant proteins ---- SCAR-like protein 1 Source.12: DFBPPR4914 ---- Plant proteins ---- Serine/threonine-protein kinase BSK1-2 Source.13: DFBPPR4974 ---- Plant proteins ---- Hsp70-Hsp90 organizing protein 1 Source.14: DFBPPR5108 ---- Plant proteins ---- Nucleoside diphosphate kinase 1 Source.15: DFBPPR5402 ---- Plant proteins ---- Nucleoside diphosphate kinase 1 Source.16: DFBPPR6280 ---- Plant proteins ---- Nucleoside diphosphate kinase 2, chloroplastic Source.17: DFBPPR6332 ---- Plant proteins ---- Nucleoside diphosphate kinase 1 Source.18: DFBPPR6478 ---- Plant proteins ---- Fructose-1,6-bisphosphatase, cytosolic Source.19: DFBPPR7495 ---- Plant proteins ---- Fructose-1,6-bisphosphatase, cytosolic Source.20: DFBPPR7617 ---- Milk proteins ---- Protein Wnt-2b Source.21: DFBPPR16016 ---- Animal proteins ---- Nitric oxide synthase, endothelial Source.22: DFBPPR16100 ---- Animal proteins ---- Nucleoside diphosphate kinase B Source.23: DFBPPR16254 ---- Animal proteins ---- Transferrin receptor protein 1 Source.24: DFBPPR16283 ---- Animal proteins ---- Nucleoside diphosphate kinase A Source.25: DFBPPR16561 ---- Animal proteins ---- UDP-glucuronosyltransferase 2B31 Source.26: DFBPPR16767 ---- Animal proteins ---- Nucleoside diphosphate kinase, mitochondrial Source.27: DFBPPR16776 ---- Animal proteins ---- Nucleoside diphosphate kinase Source.28: DFBPPR16847 ---- Animal proteins ---- Fibrinogen alpha chain Source.29: DFBPPR17017 ---- Animal proteins ---- Nucleoside diphosphate kinase A 2 Source.30: DFBPPR17048 ---- Animal proteins ---- Nitric oxide synthase, endothelial Source.31: DFBPPR17052 ---- Animal proteins ---- Nucleoside diphosphate kinase A 1 Source.32: DFBPPR17303 ---- Animal proteins ---- Phosphatidylinositol 3-kinase regulatory subunit alpha Source.33: DFBPPR17311 ---- Animal proteins ---- Nucleoside diphosphate kinase B Source.34: DFBPPR17350 ---- Animal proteins ---- DNA mismatch repair protein Msh2 Source.35: DFBPPR17739 ---- Animal proteins ---- Molybdenum cofactor biosynthesis protein 1 Source.36: DFBPPR17818 ---- Animal proteins ---- Endoribonuclease Dicer Source.37: DFBPPR17832 ---- Animal proteins ---- Myocyte-specific enhancer factor 2A Source.38: DFBPPR17900 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.39: DFBPPR17984 ---- Animal proteins ---- Phosphatidylinositol 3-kinase regulatory subunit beta Source.40: DFBPPR18859 ---- Animal proteins ---- Matrix remodeling-associated protein 8 Source.41: DFBPPR19354 ---- Animal proteins ---- Cathelicidin-7 Source.42: DFBPPR19704 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 29 Source.43: DFBPPR19984 ---- Animal proteins ---- Angiopoietin-related protein 7 Source.44: DFBPPR20096 ---- Animal proteins ---- Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial Source.45: DFBPPR20582 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 16 homolog Source.46: DFBPPR20944 ---- Animal proteins ---- Pikachurin Source.47: DFBPPR21465 ---- Animal proteins ---- Probable RNA-binding protein EIF1AD Source.48: DFBPPR21803 ---- Animal proteins ---- Uracil phosphoribosyltransferase homolog Source.49: DFBPPR22244 ---- Animal proteins ---- THAP domain-containing protein 3 Source.50: DFBPPR8528 ---- Animal proteins ---- Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial Source.51: DFBPPR8543 ---- Animal proteins ---- Sterol regulatory element-binding protein 1 Source.52: DFBPPR8583 ---- Animal proteins ---- Amiloride-sensitive amine oxidase [copper-containing] Source.53: DFBPPR8724 ---- Animal proteins ---- Nitric oxide synthase, endothelial Source.54: DFBPPR8764 ---- Animal proteins ---- Myocyte-specific enhancer factor 2A Source.55: DFBPPR8860 ---- Animal proteins ---- Nucleoside diphosphate kinase B Source.56: DFBPPR8889 ---- Animal proteins ---- Hexokinase-2 Source.57: DFBPPR9127 ---- Animal proteins ---- Transferrin receptor protein 1 Source.58: DFBPPR9133 ---- Animal proteins ---- Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial Source.59: DFBPPR10027 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1D Source.60: DFBPPR10036 ---- Animal proteins ---- Acetyl-CoA carboxylase Source.61: DFBPPR10430 ---- Animal proteins ---- Transferrin receptor protein 1 Source.62: DFBPPR10540 ---- Animal proteins ---- Nucleoside diphosphate kinase Source.63: DFBPPR10705 ---- Animal proteins ---- Endoribonuclease Dicer Source.64: DFBPPR11041 ---- Animal proteins ---- Chromodomain-helicase-DNA-binding protein 7 Source.65: DFBPPR11455 ---- Animal proteins ---- Growth factor receptor-bound protein 2 Source.66: DFBPPR11528 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 29 Source.67: DFBPPR11811 ---- Animal proteins ---- Uracil phosphoribosyltransferase homolog Source.68: DFBPPR11929 ---- Animal proteins ---- Probable RNA-binding protein EIF1AD Source.69: DFBPPR12310 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1C Source.70: DFBPPR12743 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A-like 1 Source.71: DFBPPR13151 ---- Animal proteins ---- Transferrin receptor protein 1 Source.72: DFBPPR13172 ---- Animal proteins ---- Hexokinase-2 Source.73: DFBPPR13603 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.74: DFBPPR14646 ---- Marine protein ---- Radical S-adenosyl methionine domain-containing protein 2 Source.75: DFBPPR14744 ---- Marine protein ---- Nucleoside diphosphate kinase B Source.76: DFBPPR14758 ---- Marine protein ---- Techylectin-5B Source.77: DFBPPR14903 ---- Microorganism protein ---- Transcription elongation factor SPT6 Source.78: DFBPPR15499 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 12 Source.79: DFBPPR7768 ---- Plant protein ---- Phosphoenolpyruvate carboxylase 1 Source.80: DFBPPR8247 ---- Plant protein ---- Nucleoside diphosphate kinase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	The peptide inhibited platelet aggregation and fibrinogen binding to ADP-activated platelets^[2].
Specific target protein(s)	Potential Target Protein(s): Integrin beta-3 Integrin alpha-IIb Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4 Fibrinogen gamma chain Fibrinogen alpha chain Fibrinogen beta chain Fibroleukin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Andrieux A, Hudry-Clergeon G, Ryckewaert JJ, Chapel A, Ginsberg MH, Plow EF, Marguerie G. Amino acid sequences in fibrinogen mediating its interaction with its platelet receptor, GPIIbIIIa. J Biol Chem. 1989 Jun 5;264(16):9258-65. PMID: 2722830
Other literature(s)	[1] Meddeb S, Demaret J P, Ballini J P, et al. A conformational study of Lys-Arg-Asp-Ser and analogs, a series of potent antithrombotic peptides. An approach based on simulated annealing and 1H NMR.[J]. Journal of Biomolecular Structure & Dynamics, 1994, 11(5):959. [2] Fiat A M, Migliore-Samour D, Jollã¨S P, et al. Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities[J]. Journal of Dairy Science, 1993, 76(1):301-310.
PubDate	1989